A new hybrid promoter and its expression vector in Escherichia coli.

expression of reteplase in escherichia coli top10 using arabinose promoter

Cloning and Expression of Mannheimia haemolytica PlpE Gene in Escherichia coli and its Immunogenicity Assessment

Mannheimia haemolytica is responsible for considerable economic losses to cattle, sheep, and goat industries in many parts of the world. This bacterium isone of the causative agents of shipping fever in cattle. Current vaccines against M. haemolytica are moderately efficacious since they do not provide complete protection against the disease. Production of a...

Advances and challenges in recombinant protein expression in Escherichia coli

Unusual observations during construction of a new cloning vector providing Ion gene expression in Escherichia coli

The plasmid pJMC40 containing the Ion gene was transformed into E. coil HB101 leading to clones with either pJMC40 or a large plasmid designated pJMC40ins, pJMC40ins has a 1.3 kb D N A insert outside of the Ion operon. Attempts to reduce the size of pJMC40 through circularization of a 6.7 kb EcoRI fragment containing the Ion operon and larger parts of pBR322 failed. Circularization of an 8.0 kb...

construction of a synthetically engineered nirb promoter for expression of recombinant protein in escherichia coli

background anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. the nirb promoter is the promoter of the first gene of nir operon in escherichia coli, which encodes nadh-dependent nitrite reductase. this promoter is naturally ...